Project Number: 2072-22000-041-19-I
Project Type: Interagency Reimbursable Agreement

Start Date: Mar 15, 2018
End Date: Mar 14, 2022

Objective:
1. Develop data standards for analysis of variance (ANOVA) data sets for submission of metadata and observed data in an electronically mineable format; 2. Develop a new R package with functions to retrieve data submitted following the new data standard with core functions for data analysis and meta-analysis in R; 3. Conduct a proof-of-concept analysis based on 50 datasets from Plant Disease Management Reports (PDMR) and Interregional Research Project No. 4 (IR-4).

Approach:
We obtained populations of P. ramorum from Vietnam and Taiwan with a high level of genetic diversity. We may have found the center of origin of P. ramorum. We will test the hypothesis that P. ramorum originated in Vietnam/Taiwan by conducting genotyping by sequencing (GBS) on 50 Asian strains as well as samples from Europe and the U.S. We will also determine if populations are more aggressive and have a different host range by conducting pathogenicity tests in our containment growth chamber to assess epidemic threat. A series of population genomic, evolutionary and coalescent analyses will be conducted to test the hypothesis that Asia is the center of origin of this pathogen.