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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Plant Genetic Resources and Disease Research » Research » Research Project #434362

Research Project: Rapid Ohia Death - Testing and Research Support

Location: Tropical Plant Genetic Resources and Disease Research

Project Number: 2040-21000-017-12-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Mar 1, 2018
End Date: Feb 28, 2023

Objective:
The objectives of the project are to provide additional ROD testing capacity, collect lab, greenhouse and field data, determine concentration and germination rates of ROD fungal propagules in beetle boring dust, and expand ohia resistance screening trials.

Approach:
Samples will be collected by cooperators and tested for the presence of the ROD pathogens by qPCR and/or carrot baiting. Results will be added to the ROD database. Laboratory, greenhouse and/or field environments will be utilized to evaluate and characterize disease responses. The concentrations and viability of Ceratocystis in beetle boring dust samples will be determined by dilution plating on selective media. Fungi will be identified using a combination of methods including light microscopy and qPCR. Ohia resistance screening will be conducted on ohia of diverse genotypes from uninfected stands, as well as survivors located in regions of widespread ROD mortality.