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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Research Project #434281

Research Project: Investigate the Pathogenesis of Swine Coronavirus Nonstructural Proteins

Location: Virus and Prion Research

Project Number: 5030-32000-118-17-A
Project Type: Cooperative Agreement

Start Date: May 1, 2018
End Date: Jun 30, 2019

The objective of this agreement is to rapidly investigate molecular determinant(s) of the biologic function of specific nonstructural proteins of swine coronaviruses and their potential role in development of a protective immune response. The primary focus of the research will be on the function of nonstructural protein 15 (nsp 15), a viral protein which when manipulated was shown to attenuate a coronavirus, and the evaluation of this attenuated virus in a swine challenge model for vaccine potential.

In vivo and in vitro studies will be conducted to demonstrate changes in nsp 15 will alter the function of this nonstructural protein in the pig. Loyola University will provide molecular virology expertise in coronaviruses that will include point mutations of PEDV/PDCoV and characterizing the effect of the mutation(s) on virus growth in vitro. NADC will perform in vivo studies that will include animal inoculation studies with parental and mutated PEDVs and PDCoVs to evaluate how the viruses replicate in swine and the mutated virus’ potential as vaccine prototypes.