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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Animal Metabolism-Agricultural Chemicals Research » Research » Research Project #434245

Research Project: Assessment of Soil Microbial Community Structure by Quantitative PCR Analysis

Location: Animal Metabolism-Agricultural Chemicals Research

Project Number: 3060-32420-002-009-N
Project Type: Non-Funded Cooperative Agreement

Start Date: Feb 1, 2018
End Date: Jan 31, 2019

Determine the concentration of microorganisms at the domain and phylum/class level in soil samples collected at various distances from beef production system. Soil samples from the airborne transport of antimicrobial resistant bacteria, antimicrobial resistance genes, and antibiotic residues from cattle production environments project will be utilized.

Utilizing quantitative PCR technology, soil and manure collected at various distances from a beef production facility will be analyzed for Fungi, Archaea, Bacteria, Alpha- and Betaproteobacteria, Acidobacteria, Acintobacteria, Bacteroidetes, and Firmicutes. Up to 100 soil samples will be analyzed in 20µL qPCR reactions in order to characterize the microbial community structure. Data collected from study will be used for modeling purposes and to properly direct future genetic analyses of the soil communities at beef production facilities.