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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » Research » Research Project #434220

Research Project: Characterization of the Complete Set of Prolamin Genes and Proteins Expressed in the Korean Wheat ‘Keumkang’

Location: Crop Improvement and Genetics Research

Project Number: 2030-21430-014-06-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Jan 1, 2018
End Date: Dec 31, 2020

Objective:
The objective of the research is to characterize the complete set of prolamin genes and proteins expressed in a Korean wheat cultivar.

Approach:
Targeted genome capture methods will be developed to facilitate high-throughput DNA sequencing of the prolamin gene regions from the Korean wheat ‘Keumkang’. DNA sequences will be mapped to the genome of the reference wheat ‘Chinese Spring’, annotated and deposited in public databases. Transcriptomic data will be generated from endosperm tissue from ‘Keumkang’ grain 20 days after anthesis to assess the expression levels of individual prolamin genes. A comprehensive proteomic map of total flour proteins from ‘Keumkang’ will be developed that links individual flour proteins to ‘Keumkang’ prolamin sequences, thereby integrating DNA and protein data.