Location: Reproduction Research
Project Number: 3040-31000-095-11-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Dec 16, 2017
End Date: Mar 30, 2019
Assess relationships between gilt development, sow lifetime productivity and mammary gland development and function.
Mammary gland from a subset of sows from each dietary treatment will be biopsied during lactation after each parity. Total DNA and RNA will be extracted from the mammary tissue and measured as described by Capuco et al. (2000) and Labarca and Paigen (1980). Protein content will be measured using the Lowry assay. Abundance of genes encoding for prolactin receptor (PRLR) and the milk proteins alactalbumin (LALBA) and B-casein (CSN2) will be quantified by reverse transcription quantitative PCR (RT-qPCR) using as reference genes MTG1 and MRPL39. For histological evaluation of the mammary parenchyma, sections will be embedded in paraffin and stained in Ehrlich's Hematoxylin and Eosin.