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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Research Project #434032

Research Project: Discover Sources, Genes and Develop U.S. Adapted Germplasm with Improved Protein Quantity and Quality utilizing Diversity in Cultivated

Location: Soybean Genomics & Improvement Laboratory

Project Number: 8042-21000-289-46-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2018
End Date: Sep 30, 2019

Identify and fine map new major QTL controlling protein concentration in wild that have not been discovered and can be used to improve the cultivated soybean. Characterize high yield, high protein lines selected by breeders with genetic variants or genetic markers.

Based upon the analysis of the nearly 1,200 wild soybean accessions in the USDA Soybean Germplasm Collection that have been analyzed with more than 50,000 SNP DNA markers a core collection of 81 wild soybeans were identified. F6 seeds were obtained by crossing the wild soybean accessions of the core subset with elite soybean cultivars by collaborators. The F6-derived recombinant inbred lines (RILs) from 10 cultivated x wild crosses will be genotyped with BARCSoySNP5K/Soysnp50k BeadChips or sequencing. The RILs will be evaluated for protein content and other seed compositions in two years. The genotypic and phenotypic data will be used to identify loci and haplotypes that are associated with high protein content and are specific in wild accessions. The identified genes will be introduced to elite cultivated soybean by collaborators. Collaborators in this project will develop new soybean lines. They will test the lines in local and regional trials and to identify high protein lines that are equivalent in yield of the best cultivars available. Genetic genotypic data will be collected from these lines. The information will be used to determine uniqueness of the lines compared to the soybean accessions in the USDA-ARS Germplasm Collection or breeding programs, and to identify regions of the genome that must be present in order to produce high protein and yielding lines. Subsequently, markers will be developed and be used to assist gene transfer in the breeding programs.