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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Research Project #434001

Research Project: Determining Genetic Factors that Influence Forage Quality in Alfalfa (Medicago sativa L.)

Location: Plant Germplasm Introduction and Testing Research

Project Number: 2090-21000-036-07-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2017
End Date: Aug 31, 2020

Objective 1. Identify 200 diverse genotypes from private industry for use in the experiment. Objective 2. Determine quality of these genotypes for first cutting at four locations in the PNW. Oblective 3. Identify molecular markers and genes that influence fiber quality (NDF, NDFD32, NDFD48 ,Kd, uNDF, TTNDFD) in alfalfa.

A diverse panel of alfalfa populations will be used for genome-wide association mapping to identify molecular markers associated with fiber quality traits. A genotyping-by-sequencing (GBS) will be sued for developing thousands of markers throughout the genome. Single nucleotide polymorphism (SNP) will be mapped and HapMap containing SNP sites and subjected to genome-wide association analysis in combination with phenotypic data of fiber quality traits.