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ARS Home » Plains Area » College Station, Texas » Southern Plains Agricultural Research Center » Food and Feed Safety Research » Research » Research Project #433896

Research Project: Global Immuno-Metabolism Kinome Profile and Immunological Analysis of Broilers Administered AviPlus®

Location: Food and Feed Safety Research

Project Number: 3091-32000-035-61-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Jan 1, 2018
End Date: Jun 30, 2021

Objective:
1. Determine the immunological effects on in vitro heterophil and monocyte function in broiler chicks fed a diet supplemented with AviPlus (0, 300, and 500 g/metric ton). 2. Determine the global kinome profile of intestinal samples collected from broilers fed a diet supplemented with AviPlus (0, 300, and 500 g/metric ton) using an immuno-metabolism peptide array. 3. Evaluate the effect of AviPlusP® supplementation (0 and 500 g/metric ton) in a coccidia/necrotic enteritis (NE) model using Coccivac® and Clostridium perfringens challenges in broiler chickens.

Approach:
Determine the immunological effects on in vitro heterophil and monocyte function in broiler chicks fed a diet supplemented with AviPlus® (0, 300, and 500 g/metric ton). Day-old chicks will be randomized and placed into three treatment groups, two replicate pens per treatment: Treatment 1: Control, feed does not contain AviPlus®; Treatment 2: Feed contains 300 g/metric ton AviPlus®; Treatment 3: Feed contains 500 g/metric ton AviPlus®. At four- and 14-days-of-age, blood will be collected and heterophils and monocytes isolated. Key immunological assays will be performed to determine if AviPlus® has an effect on in vitro heterophil/monocyte function including degranulation, oxidative burst, and cytokine/chemokine mRNA expression levels. Determine the global kinome profile of intestinal samples collected from broilers fed a diet supplemented with AviPlus® (0, 300, and 500 g/metric ton) using an immuno-metabolism peptide array. Day-old chicks will be randomized and placed into three treatment groups, two replicate pens per treatment: Treatment 1: Control, feed does not contain AviPlus®; Treatment 2: Feed contains 300 g/metric ton AviPlus®; Treatment 3: Feed contains 500 g/metric ton AviPlus®. At day of hatch, 5 chicks will be sacrificed for baseline controls. At 15 days-of-age, birds (n=5) from each pen will be sacrificed and a section of ileum and jejunum will be collected and analyzed using a peptide array to determine the global kinome profile. Evaluate the effect of AviPlusP® supplementation (0 and 500 g/metric ton) in a coccidia/necrotic enteritis (NE) model using Coccivac® and Clostridium perfringens challenges in broiler chickens. Part I: Determine if AviPlusP® (0 and 500 g/MT) affects gross intestinal lesion scores and/or the number of recoverable C. perfringens. Part II: Determine the global kinome profile of intestinal tissues using the immuno-metabolism peptide array. Sections of ileum, jejunum, and duodenum will be collected, and depending on lesion distributions we will decide which tissues to analyze with the peptide array. Serum and fecal samples will also be collected and subjected to an ELISA to measure iFABP and Calprotectin, respectively. Day-old chicks will be randomized and placed into eight treatment groups (Table 1). These studies will provide valuable data showing if AviPlusP® can reduce intestinal lesions associated with coccidia and/or NE, two of the costliest diseases facing the poultry industry, as well as provide insights into the mode of action. This experiment will be conducted on two occasions and will terminate at approximately 21 days post hatch.