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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Sunflower and Plant Biology Research » Research » Research Project #433862

Research Project: Applying Genomic Tools to Accelerate Breeding for Disease Resistance in Confection Sunflower

Location: Sunflower and Plant Biology Research

Project Number: 3060-21000-043-14-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Oct 1, 2017
End Date: Sep 30, 2019

Objective:
Objective 1: Analyze allelic relationship of the DM R gene Pl17 with a new gene Plann using a large F2 segregation population (1,000-2,000 F2 individuals) derived from the cross between Pl17 and Plann. Objective 2: Conduct high-resolution genetic and physical maps of the three DM R-genes, Pl17, Pl18, and Plann using a fine mapping strategy. Objective 3: Identify candidate genes for DM resistance. Objective 4: Develop user-friendly ‘perfect’ markers for DM R genes and release these markers to the sunflower breeding industry for MAS to rapidly incorporate the DM R-genes into finished commercial hybrids.

Approach:
We will conduct a highly integrated project that involves the following components: 1) Allelism test for DM resistance in HA 458 (Pl17) and a line carrying Plann. Cross will be made and a large F2 population (1,000-2,000 F2 individuals) will be phenotyped for DM responses in the greenhouses, 2) Fine mapping of DM R genes, Pl17, Pl18, and Plann, and placement of these genes to specific sequence scaffold(s), 3) Identification of candidate genes underlying each DM R genes. Functional information inferred from other species will be used to identify candidate genes. The genetic position of the DM R genes relative to candidate genes will be refined by selective genotyping and phenotyping of recombinants in the DM R gene regions, 4) Sequence analysis of DM R gene regions from the R gene donor lines. This sequence information will be used to design additional assays for the segregation of SNPs in candidate genes, 5) Development and application of SNP markers for marker-assisted selection, and 6) Interactions between DM pathogen and sunflower host.