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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Research Project #433824

Research Project: Comparative Genomic and Virulence Analysis of Streptococcus Suis Isolates

Location: Virus and Prion Research

Project Number: 5030-32000-119-11-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 16, 2017
End Date: Nov 30, 2018

Objective:
1) Obtain closed whole-genome sequences of S. suis isolates that are known to exhibit different pathogenic capacities to perform comparative genomic analyses. 2) Perform RNA sequencing of S. suis isolates to generate a more comprehensive assessment of the adaptive transcriptional response of these strains. 3) Use in vitro assays (adherence, invasion, oxidative stress, and biofilm assays) to test the capacity of these strains to adhere, invade, survive and/or persist within conditions that mimic various host microenvironments.

Approach:
We plan to obtain DNA sequencing and RNA sequencing data on isolates that harbor unique serotype types and virulence capacities to perform comparative genomic analyses and evaluate adaptive transcriptional responses. This analysis should illuminate genes that are present, absent, and/or contain nucleotide differences among S. suis isolates that are known to exhibit different pathogenic capacities subsequently providing genotypic links to virulence. Additionally, we plan to perform a variety of in vitro assays test the capacity of these strains to adhere, invade, survive and/or persist within conditions that mimic various host microenvironments.