Project Number: 2034-22000-012-13-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Oct 1, 2017
End Date: Dec 31, 2019
To use electropenetrography (EPG) to identify the amount/types of feeding by blue-green sharpshooters on grapevine plants treated with residual insecticides, especially specific behaviors that control acquisition and inoculation of Xylella fastidiosa.
Insecticide BYI 02960 will be applied to host plants at a rate equivalent to that used in the field. Four blue-green sharpshooters will be attached to gold-wire tethers and their feeding EPG-recorded for 20 hours each on one of four treatments:three residual/holding times (90 minutes, 3 or 7 days) for insecticide-treated plants, plus an untreated control. The experimental design will be a randomized complete block replicated 20 times. Recordings will be interpreted for behaviors performed; durations of those behaviors will be quantified. Results will be statistically analyzed using mixed-model ANOVA and least significant difference tests. Deliverables will include effects of insecticides on overall feeding and performance of specific behaviors responsible for acquisition or inoculations of Xylella fastidiosa.