Location: Foreign Disease-Weed Science Research
Project Number: 8044-22000-046-10-I
Project Type: Interagency Reimbursable Agreement
Start Date: Aug 1, 2017
End Date: Sep 30, 2018
To generate specific immunoreagents and develop an immunoassay for detection of boxwood blight in infected hosts.
We will identify a set of candidate Calonectria proteins and peptides suitable for targets of immuno- assays. Promising candidate proteins and/or peptides will be used as immunogens to generate monoclonal antibodies (mAbs), using a commercial provider. We will test mAbs for sensitivity against the target antigen in pre-symptomatic samples, and during the course of the infection cycle, again using mock diagnostic samples in ELISA format. We will use the mAbs to test applicability and reactivity in mock diagnostic samples, extracted using field extraction buffer kits with leaf samples. We will also test the specificity against near neighbors and boxwood-associated pathogens and endophytes. All testing using Calonectria henricotiae will take place within BL3 containment at the ARS facility in Frederick MD. Promising candidate PAb and MAb immunodiagnostic reagents will be licensed to commercial diagnostic companies through an ARS biological materials license.