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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Plant Pathology Research » Research » Research Project #433628

Research Project: Collaborative Research to Control Thrips and Tospoviruses

Location: Subtropical Plant Pathology Research

Project Number: 6034-22000-042-022-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Aug 1, 2017
End Date: Jul 31, 2022

Objective:
Develop novel means for control of thrips and tospoviruses.

Approach:
Previous work has demonstrated that a protein on the surface of tomato spotted wilt virus binds to a thrips gut receptor to facilitate virus entry and replication. It is also known that the specificity of Bt toxin depends on targeting Bt to an insect gut receptor by a specific protein. We are using standard molecular techniques to produce Bt that contains the virus surface protein in place of its native targeting protein. The rationale is that the Bt fusion will bind to thrips guts (and only thrips guts because the virus is not transmitted by any other insect) and create a specific (no harm to beneficial insects), benign (years of use of Bt have shown it to be safe) and effective pesticide. A robust transmission assay will be used to experimentally evaluate Bt fusions for control of thrips and tospoviruses.