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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research » Research » Research Project #433617

Research Project: Inducement of Malolactic Fermentation Using Lactobacillus plantarum in Musts and Wines from the Pacific Northwest

Location: Horticultural Crops Research

Project Number: 2072-21000-057-04-G
Project Type: Grant

Start Date: Sep 1, 2017
End Date: Mar 31, 2020

1. Screen native strains of Lactobacillus plantarum previously isolated from wines for glycosidase, phenolic acid decarboxylase, biogenic amine synthesis and other activities important for wine quality. 2. Investigate interactions between Saccharomyces cerevisiae and Lactobacillus plantarum.

Strains of Lactobacillus plantarum originally isolated from regional wines and commercially-available will be used in this research. In addition, a strain of Lb. brevis WS-21B, as well as commercial strains of Oenococcus oeni will be utilized. Bacteria will initially be characterized regarding such enzymatic activities as glycosidase (desirable) and phenolic acid decarboxylase (undesirable) and others using established methods. For fermentations, Cabernet Sauvignon grapes will be obtained and processed using standard winemaking protocols. Grape musts will be inoculated with a yeast commonly used in red fermentations. Bacterial inoculations will occur (a) at yeast inoculation and (b) after completion of alcoholic fermentation. Cell growth, progress of alcoholic and malolactic fermentations, and synthesis of volatile compounds such as higher alcohols, esters, diacetyl, and others important for quality will be measured.