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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research » Research » Research Project #433578

Research Project: Developing Tetraploid Forms of Maples to Breed for Reduced Invasiveness

Location: Horticultural Crops Research

Project Number: 2072-21220-003-22-G
Project Type: Grant

Start Date: Sep 1, 2017
End Date: Sep 30, 2019

Objective:
Develop tetraploid forms of economically important maple species that will be used to breed for triploids that have reduced fertility, and thus less invasiveness.

Approach:
Tetraploids will be induced using methods that have proven successful for Amur, Norway, and trident maples. Seeds will be collected from superior clones of Acer campestre (hedge maple), A. davidii (snakebark maple), A. griseum (paperbark maple), and A. pseudoplatanus (sycamore maple). Seed will be cold-stratified and then sown in soilless substrate in flats in a glasshouse and treated upon emergence. Surviving seedlings will be transplanted and accessioned and then tested for ploidy level using flow cytometry, which is the greatest percentage of time/labor required on the project. In order to confirm flow cytometry results, chromosome counts will be performed such that genome size data can be used to infer ploidy level.