Location: Vegetable Crops Research
Project Number: 5090-21000-066-002-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2017
End Date: Aug 31, 2019
Objective:
Screening potato germplasm for resistance to Dickeya dianthicola and associated work.
Approach:
Tubers will be generated from each line and stab inoculated with D. dianthicola. Up to 5 tubers per line will be inoculated, tubers will be incubated for 3 days at 28C in low-oxygen conditions and rated on a 1-3 scale for symptoms, with 1 = no symptoms, 2 = mild symptoms, and 3 = severe soft rot. The lines with the highest apparent resistance will be rescreened to confirm the results.
Based on previous results, we expect to identify highly resistant and highly susceptible lines within each species. Stems and tubers from a subset of highly resistant and highly susceptible lines will be used for proteomic and metabolomic analysis to determine if metabolites or proteins correlate with resistance can be identified. Antimicrobial peptides associated with resistance to Pectobacterium have been found in potato and we will also specifically assay for these peptides. We already have data for metabolites and proteins found in the stem of a Pectobacterium-resistant S. chacoense line and a Pectobacterium-susceptible S. phureja line, as well as in tubers of 60 commercial varieties or elite breeding lines for comparison. Data on accession responses, metabolites, and proteins identified will be made available for other breeders.
