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ARS Home » Plains Area » Lincoln, Nebraska » Agroecosystem Management Research » Research » Research Project #433484

Research Project: Single Nucleotide Discovery and Preliminary Population Genetics for the Stable Fly, Stomoxys Calcitrans

Location: Agroecosystem Management Research

Project Number: 3042-32000-011-05-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 15, 2017
End Date: Mar 31, 2021

1) To discover the single nucleotide polymorphisms (SNPs) in the stable fly from different regions of the United States (U.S.) 2) To conduct a preliminary population genetics study on U.S. stable flies using the discovered SNPs.

ARS scientists collected and phenotyped stable flies from throughout the U.S. The Cooperator Scientist will extract DNA, genotype flies, identify phenotypic and geographic distinct SNPs and conduct a preliminary population genetics study using the provided samples. We will use next-generation sequencing approaches (genome-by-sequencing) for SNP discovery on a minimum of 75 flies. The current collection consists of male and female flies from eight locations, representing two phenotypes (8 locations x 2 sexes x 2 phenotypes x >2reps = >64 specimens for sequencing and analyses).