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ARS Home » Plains Area » Lincoln, Nebraska » Agroecosystem Management Research » Research » Research Project #433484

Research Project: Single Nucleotide Discovery and Preliminary Population Genetics for the Stable Fly, Stomoxys Calcitrans

Location: Agroecosystem Management Research

Project Number: 3042-32000-010-11-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 15, 2017
End Date: Sep 14, 2020

Objective:
1) To discover the single nucleotide polymorphisms (SNPs) in the stable fly from different regions of the United States (U.S.) 2) To conduct a preliminary population genetics study on U.S. stable flies using the discovered SNPs.

Approach:
ARS scientists collected and phenotyped stable flies from throughout the U.S. The Cooperator Scientist will extract DNA, genotype flies, identify phenotypic and geographic distinct SNPs and conduct a preliminary population genetics study using the provided samples. We will use next-generation sequencing approaches (genome-by-sequencing) for SNP discovery on a minimum of 75 flies. The current collection consists of male and female flies from eight locations, representing two phenotypes (8 locations x 2 sexes x 2 phenotypes x >2reps = >64 specimens for sequencing and analyses).