Skip to main content
ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Genetic Improvement for Fruits & Vegetables Laboratory » Research » Research Project #433475

Research Project: Development of SNP Markers for a Diploid Potato Population

Location: Genetic Improvement for Fruits & Vegetables Laboratory

Project Number: 8042-21000-273-26-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2017
End Date: Sep 30, 2018

Objective:
To develop molecular markers for a diploid potato population segregating for traits associated with nitrogen uptake efficiency, tuberization response under long days, yield, and specific gravity using single nucleotide polymorphisms (SNPs).

Approach:
Leaf material will be collected from 6-week-old plants from a population of 200+ clones from a Solanum phureja-S. stenotomum x S. chacoense cross, frozen in liquid nitrogen and freeze dried. Total genomic DNA will be extracted and purified from dried potato leaf tissue using the DNeasy Plant Mini Kit (QIAGEN). Genotyping will be performed by using the Illumina Solanaceae Coordinated Agricultural Project (SolCAP) potato single-nucleotide polymorphism (SNP) array (Neogen Corporation and Michigan State University). Genome loci significantly associated with nitrogen uptake efficiency, tuberization response under long days, yield and specific gravity will be identified. SNPs linked to these traits will be identified and will be used as markers for future selection.