Project Number: 8042-21000-283-07-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2017
End Date: Sep 30, 2018
To develop molecular markers for a diploid potato population segregating for traits associated with nitrogen uptake efficiency, tuberization response under long days, yield, and specific gravity using single nucleotide polymorphisms (SNPs).
Leaf material will be collected from 6-week-old plants from a population of 200+ clones from a Solanum phureja-S. stenotomum x S. chacoense cross, frozen in liquid nitrogen and freeze dried. Total genomic DNA will be extracted and purified from dried potato leaf tissue using the DNeasy Plant Mini Kit (QIAGEN). Genotyping will be performed by using the Illumina Solanaceae Coordinated Agricultural Project (SolCAP) potato single-nucleotide polymorphism (SNP) array (Neogen Corporation and Michigan State University). Genome loci significantly associated with nitrogen uptake efficiency, tuberization response under long days, yield and specific gravity will be identified. SNPs linked to these traits will be identified and will be used as markers for future selection.