Project Number: 6028-31630-008-06-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2017
End Date: Jun 30, 2019
Under the guidance of the ARS PI, the university MS student will, formulate and feed test diets that maximize fish meal (FM) replacement with three commercial protein concentrates in hybrid striped bass (HSB) in replicated tank trials, and determine growth, body/fillet composition, and non-specific immune responses to the three concentrates in comparison to fish fed a FM-based reference diet.
To determine the biological response to the concentrates of interest a completely randomized design growth trial will be conducted using a fish meal (FM)-based reference diet. The experimental variable in the growth trials will be protein level (60, 65, or 70%) of the Aqua™ product line on a digestible protein basis. All experimental diets will provide 40% Digestible Protein and 16% lipid and will meet nutritional requirements of HSB. Fish will be stocked at 20-30/tank in three replicate tanks/diet and fed to satiation once daily for 8-12 weeks. Fish will be weighed periodically to track growth. At the trial conclusion a subset of 5 fish will be sampled from each tank, bled to conduct health assays and then ground for biochemical analysis of nutrient, energy, and amino acid retentions. A second set of 5 fish from each tank will be sampled for bioindices that include hepatosomatic index (HSI), intraperitoneal fat (IPF) ratio, and muscle ratio. Data will be analyzed using a factorial ANOVA with Fisher’s least significant difference test to identify specific differences among treatment means (Significance, P<0.05). Responses will be also analyzed via orthogonal contrasts to determine if there are differences in mean responses to the protein concentrates in comparison to the FM control diet, and linear contrasts to determine if responses vary among protein concentrates.