Location: Vegetable Crops Research
Project Number: 5090-21220-005-014-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2017
End Date: Aug 31, 2020
Objective:
Determine candidate neonicotinoid resistance genes in populations of Colorado potato beetle (CPB)
Approach:
Population genomic analyses will be performed to compare resistant and susceptible CPB populations. Gene expression data will be integrated to identify putative gene networks underlying resistance.
RNAseq data will be analyzed for up-regulation of specific genes in resistant vs. susceptible CPB populations.
Select candidate resistance genes will be tested for phenotypic effects using RNAi knockdown experiments.