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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Mosquito and Fly Research » Research » Research Project #433269

Research Project: Aedes Surveillance, Pathogen Testing and Host Preference Study in Caribbean/Central America

Location: Mosquito and Fly Research

Project Number: 6036-32000-050-69-I
Project Type: Interagency Reimbursable Agreement

Start Date: Mar 2, 2017
End Date: Apr 28, 2018

Determine the distributions, infection rates, and host biting preference among Aedes aegypti and Aedes albopictus and their impact on transmission of Chikungunya and dengue in rural and urban areas at select sites in the Southern Command Area of Responsibility (Dominican Republic, Haiti, Trinidad and Tobago, Guatemala).

Passive adult mosquito trapping techniques will be conducted using BG-SentinelTM traps (Biogents AG, Regensburg, Germany) with lure. We estimate that 10 mosquito traps will be deployed to each site and set three 24 hr periods per week over a six month sample period. BG-Sentinel traps will be positioned peripheral to (rural) and within communities (urban) using indoor and outdoor sampling pooling to determine Aedes aegypti and Aedes albopictus prevalence. Traps will be set at sunset and serviced (i.e., change catch bags, connect charged battery) daily. Mosquito samples will initially be tested for the presence of Chikungunya and dengue antigens using VecTest kits (Vector Test Systems, Thousand Oaks, California). Mosquito-supernatant will then be preserved, shipped, and analyzed using real time polymerase chain reaction analysis at either the University of Florida lab (Trinidad and Tobago, Haiti, and Dominican Republic sites) or NEPMU-2 (Guatemala). In addition, the blood feeding preferences of the mosquitoes will be determined in Aedes aegypti and Aedes albopictus. Wild caught blood-fed Aedes albopictus and Aedes aegypti in the 4 Southern Command Area of Responsibility countries will be shipped to the University of Florida where mosquitoes will be tested for human blood and other vertebrate blood using a polymerase chain reaction based methods. Surveillance activities will continue during the rainy season and into the dry season to determine the influences of weather in mosquito abundance, pathogen infection, and host blood meals. Global Positioning Systems (GPS) and Geographic Information Systems (GIS) will be used to keep records of surveyed areas, location of traps, and other relevant geographical information favorable for vector populations. Surveillance data will be systematically collected (i.e., adult Aedes individuals will be identified and counted per trap) following guidance in [24] to be able to assess relative densities of species at particular trap locations. If positive Chikungunya or dengue mosquitoes are identified, host nation public health officials will be notified. All surveillance and disease data will be input into VectorMap. Surveillance protocols and data capture will be similar across all field sites.