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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Research Project #433246

Research Project: The Population Genetics of Lygus Hesperus in Alfalfa Seed-Production Fields

Location: Vegetable Crops Research

Project Number: 5090-21000-068-07-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 20, 2017
End Date: Sep 19, 2019

The objective is to examine the genetic diversity and population genetic structure of a major pest in alfalfa-seed production fields, Lygus hesperus pre and post insecticide use.

30 Lygus hesperus individuals from each of 3 populations will be collected in each of two geographic areas, the Pacific Northwest and the Central Valley of California both before and after insecticide spray. Deoxyribonucleic acid (DNA) and Genotyping by Sequencing (GBS) will be performed on the samples. Gene frequencies will be compared among populations and among geographic regions, both pre and post spray. This information will be used to determine whether there exists genetic differentiation in Lygus hesperus populations over alfalfa seed-production fields and whether and how gene frequencies are affected by spraying.