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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Genetic Improvement for Fruits & Vegetables Laboratory » Research » Research Project #433229

Research Project: Targeting Cranberry Fruit Chemistry to Develop Cultivars with Novel Phytochemical Profiles for Healthier, Reduced ‘Added-Sugar’ Products

Location: Genetic Improvement for Fruits & Vegetables Laboratory

Project Number: 8042-21000-279-02-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Mar 15, 2017
End Date: Mar 14, 2021

Objective:
1. Complete reference quality genome for cranberry. 2. Fine map cranberry using GBS (genotyping by sequencing). 3. Phenotype cranberry for fruit traits. 4. Phenotype cranberry for fruit chemistry. 5. Map QTL (quantitative trait loci) associated with desired traits. 6. Find key genes associated with selected QTL.

Approach:
1. Combine existing next-generation sequencing data with ‘new’ long-read data (i.e. PacBio data) to improve the current draft genome. 2. Add GBS markers to existing maps to increase marker density. 3. Phenotype fruit from selected crosses for ripening time, color, size, fruit rot, and etc. 4. Phenotype fruit chemistry, primarily by HPLC, for organic acids, flavonoids, phenolics, etc. that may impact human health. 5. Map the desired traits using GBS on segregating populations. 6. Use the map data and reference genome to ‘find’ the genes responsible for selected QTL.