Location: Sugarbeet and Potato Research
Project Number: 3060-21430-007-07-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 1, 2017
End Date: Sep 30, 2019
To determine: 1) the efficacy of chitosan oligosaccharide (COS) and its derivatives as treatments to elicit rapid WH responses in cut seed and in potatoes entering storage, 2) the role(s) of protective redox-linked pentose phosphate pathway (PPP) regulation in potentially improving WH and suberization processes in cut seed tubers, 3) the efficacy of natural elicitors as treatments to elicit rapid WH responses in cut seed and bruised potatoes entering storage, and 4) the role(s) of protective redox-linked PPP and associated proline metabolism in improving WH and suberization processes in cut seed and bruised potato tubers.
A replicated laboratory trial will be conducted with standard statistical design at USDA-ARS Sugarbeet and Potato Research Unit and at NDSU Plant Science laboratory in Fargo, ND. Certified seed tuber potatoes will be collected and a disc model system will be used for tuber tissue WH systems (Lulai et al., 2008). Different concentrations (0, 10, 25, 50, & 100 ppm) and formulations of COS (ascorbic acid, cran-oligo), proline and proline analogs will be tested as treatments to elicit rapid WH responses of cut seed tubers. Objectives 1 and 3.: Suberization ratings will be histochemically determined in blocks of tissue from treated and untreated tuber discs (Lulai et al., 2008). Accumulation of SPP and SPA will be determined using method described by Lulai et al. (2006). Water vapor loss (shrinkage) from cut tubers and PAL activity will also be determined (Lulai et al., 2008). Objectives 2 and 4.: Activity of different enzymes associated with anabolic PPP regulation such as glucose-6-phosphate dehydrogenase, succinate dehydrogenase, proline dehydrogenase and proline content of cut potato tubers with and without natural elicitor treatments will be determined spectrophotometrically (Sarkar et al., 2010). Total phenolic content, phenolic profile (HPLC), antioxidant enzyme (catalase, peroxidase, superoxide dismutase) activity will also be determined spectrophotometrically (Sarkar et al., 2010). Data will be analyzed using Statistical Analytical Systems (SAS) using a standard statistical model.