Project Number: 5020-21000-008-03-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 1, 2017
End Date: Jul 31, 2019
1) Understand the infection process of soybean sudden death syndrome and factors affecting this process; and 2) Understand the roles of combination of toxins in SDS development and identify novel toxins.
1. Generate GFP-tagged mutant of Fusarium virguliforme; use it to infect SDS-susceptible and SDS-resistant soybean cultivars; monitor infection process with fluorescent microscopy and molecular techniques. 2. Generate mono-, double- and triple- mutants of F. virguliforme in which one or more known or suspected toxin genes are deleted, and use the mutants to infect soybean.