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ARS Home » Research » Research Project #433043

Research Project: FHB Management in Barley: QTL Deployment and Phenotyping

Location: National Programs

Project Number: 0500-00053-004-28-G
Project Type: Grant

Start Date: Jul 12, 2017
End Date: Jul 11, 2021

Objective:
(1) Map a putative new source of DON accumulation resistance in a Turkish two-row line. (2) a) To Screen elite 2-row and 6-row barley germplasm for resistance to Fusarium head blight (FHB) in iniform screening nurseries in North Dakota; b) To coordinate and carryout distribution of germplam bewteen collaborating groups in upper Midwestern United States and two breeding programs in Canada so that germplam can be tested in trials across North America; and c) To receive the reports of the Collaboarint groups, collate, and interpret them and make them freely available to all interested parties including posting on the USWBSI's website.

Approach:
(1) These populations will be tested over the next two years in the field at three locations, acquire DON accumulation data and perform the genotyping of the population using at least 384 SNPs from several PCR-GBS panels that we have developed for Ion Torrent sequencing technology. QTL analyses will be performed to identify DON accumulation QTL and associated markers. (2) The cooperative nursery is called the North American Barley Scab Evaluation Nursery (NABSEN). The NABSEN will contain the most advanced barley lines with putative FHB resistance and will be grown in eight locations across the region where FHB is affecting the crop. There will be a mix of locations for the NABSEN, with some being mist irrigated and inoculated and others grown under natural environmental conditions. Data will be collected on FHB severity, FHB incidence and heading date. Samples will also be tested for DON content.