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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Meat Safety & Quality Research » Research » Research Project #432920

Research Project: Antimicrobial Resistance Risk Assessment Data Gaps - Retail Beef

Location: Meat Safety & Quality Research

Project Number: 3040-42000-018-06-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Jun 1, 2017
End Date: Aug 31, 2018

Objective:
Determine relative abundance and extent of AMR in retail beef products, using metagenomic, culture and qPCR approaches.

Approach:
Over the course of approximately 10 months (to insure a representative seasonal sample population), collect a total of 600 retail ground beef samples from 5 stores in each of 6 major U.S. metropolitan cities (Denver, Chicago, Los Angeles, Houston, New York, Atlanta). In each store sampled, 10 samples of unassociated (i.e., from differing fat percentages, labels, etc.) Natural and 10 samples of Conventional ground beef will be collected (20 samples/store/city). Samples will be composited by type of product (i.e., Natural vs. Conventional) and Store (a total of 60 composites reflecting 30 stores x 2 types of beef composited/store). Samples will be cultured to determine the prevalences and concentrations of antimicrobial resistant bacteria. DNA will be extracted each sample to determine presence of antimicrobial resistance genes.