Location: Foreign Animal Disease Research
Project Number: 8064-32000-060-14-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jul 1, 2017
End Date: Jun 30, 2019
The objective of this research project is to evaluate Classical Swine Fever Virus (CSFV) vaccine strains developed at ARS, PIADC or CReSA IRTA throughout genomic manipulation by deleting specific virus genes involved in virulence in swine. Specific objectives include: 1. Vaccine strains will be assessed for their immunogenicity and particularly their ability to induce protection against the challenge with virulent virus stains. 2. Immunological studies will be performed towards the identification of immune mechanisms mediating protection against disease.
Viruses will be developed by ARS, PIADC and tested in vivo at CReSA. Swine will be innoculated with Classical Swine Fever Virus (CSFV)vaccines. The level of protection will be assessed by recording the appearance of CSF-related clinical signs as well as the presence of challenge virus in clinical samples. Presence of challenge virus in blood and clinical samples will be quantified by detecting infectious virus as well as by quantitative real time PCR. Presence of virus specific immune response will be assessed by detecting ELISA and gamma interferon producing cells.