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ARS Home » Plains Area » College Station, Texas » Southern Plains Agricultural Research Center » Crop Germplasm Research » Research » Research Project #432794

Research Project: Coordinated Development of Genetic Tools for Pecan

Location: Crop Germplasm Research

Project Number: 3091-21000-039-03-A
Project Type: Cooperative Agreement

Start Date: Oct 1, 2016
End Date: May 31, 2021

Objective:
Work within a coordinated national team of researchers to develop molecular markers useful in pecan breeding and selection, specifically working to refine markers related to cultivar identity verification and pecan scab disease resistance.

Approach:
Cooperator will be responsible for conducting digital genotyping, a form of genotyping by sequencing, on several pecan mapping populations. Graduate and student workers will be responsible for DNA extraction as well as preparing Illumina libraries for sequencing on an Illumina HiSeq2500 from 3 pecan mapping populations consisting of ~800 trees. The students will also be responsible for genetic map construction using the SNP data obtained from digital genotyping and will work to map traits of interest with an initial focus on scab resistance QTL. Cooperator will also conduct additional marker development for cultivar identity verification using both microsatellite and SNP analysis.