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ARS Home » Northeast Area » Orono, Maine » New England Plant, Soil and Water Research Laboratory » Research » Research Project #432774

Research Project: Advancing the Technologies in Detecting, Diagnosing, and Controlling Black Leg Disease (Dickeya spp.) in the U.S.

Location: New England Plant, Soil and Water Research Laboratory

Project Number: 8030-12210-001-03-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Apr 14, 2017
End Date: Oct 14, 2018

Objective:
1) Determine the genetic background and distribution of Dickeya and Pectobacterium isolates. 2) Determine relative virulence of Pectobacterium and Dickeya isolates on potato varieties.

Approach:
Obj. 1) A survey of seed potatoes from Wisconsin will be conducted. Soft rot bacteria will be isolated from the stem ends of tubers and from stems of field grown plants. Isolates will be stored in glycerol stocks at -80C. Each isolate will be identified to species or subspecies level with a combination of molecular and biochemical tests. Depending on the results obtained, a multi-locus sequence analysis based on several housekeeping genes will be made as previously described (Ma et al., 2007; Kim et al., 2008). Obj. 2) A novel video analysis system will be used to rate disease in inoculated stems from commercial varieties. Tubers from important commercial varieties and from tolerant wild species or breeding lines will be inoculated with strains isolated from Wisconsin to determine relative susceptibility to the soft rot species isolated from Wisconsin.