Project Number: 2034-22000-013-09-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Oct 1, 2017
End Date: Sep 30, 2020
Objective 1. Characterize MCA13-CTV (MCA13-Reactive Citrus Tristeza Virus) from Central California and compare with mild and severe CTV isolates from Peru which uses cross-protection to mitigate severe CTV-induced disease. Objective 2. Examine temporal and spatial spread of MCA13-CTV isolates in central California. Objective 3. Examine effects of MCA13-CTV on citrus physiology and symptom expression.
Conduct virus indexing of CTV isolates in greenhouse to document disease virulence, collect tissue and analyze for amino acid, carbohydrate, phenolic and terpenoid compounds. Purify small interfering RNAs from infected citrus and outsource for Next Generation Sequencing, assemble and align contigs, fill sequence gaps by overlapping primer sets, produce polymerase chain reaction (PCR) products, cloning and sequencing. Monitor CTV-infected tree incidence by enzyme-linked immunosorbent assay (ELISA) and map infected trees and analyze spread pattern by Monte Carlo and other statistical tests or algorithms.