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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Research Project #432748

Research Project: Assessing Impact MCA13-Reactive Citrus Tristeza Virus Isolates in Tulare County Pest Control District Orchards

Location: Crop Diseases, Pests and Genetics Research

Project Number: 2034-22000-013-09-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2017
End Date: Sep 30, 2019

Objective:
Objective 1. Characterize MCA13-CTV (MCA13-Reactive Citrus Tristeza Virus) from Central California and compare with mild and severe CTV isolates from Peru which uses cross-protection to mitigate severe CTV-induced disease. Objective 2. Examine temporal and spatial spread of MCA13-CTV isolates in central California. Objective 3. Examine effects of MCA13-CTV on citrus physiology and symptom expression.

Approach:
Conduct virus indexing of CTV isolates in greenhouse to document disease virulence, collect tissue and analyze for amino acid, carbohydrate, phenolic and terpenoid compounds. Purify small interfering RNAs from infected citrus and outsource for Next Generation Sequencing, assemble and align contigs, fill sequence gaps by overlapping primer sets, produce polymerase chain reaction (PCR) products, cloning and sequencing. Monitor CTV-infected tree incidence by enzyme-linked immunosorbent assay (ELISA) and map infected trees and analyze spread pattern by Monte Carlo and other statistical tests or algorithms.