Location: Plant, Soil and Nutrition Research
Project Number: 8062-52000-003-004-I
Project Type: Interagency Reimbursable Agreement
Start Date: Mar 15, 2017
End Date: Mar 14, 2022
Objective:
Objective 1: Establish and asses the genetic diversity of a panel of 200 yellow bean genotypes from around the world of both Andean and Middle American origin.
Objective 2: Phenotype the Yellow Bean Diversity Panel for agronomic value, convenience, nutrition and taste.
Objective 3: Phenotype and conduct QTL (Quantitative Trait Loci) mapping for iron bioavailability and cooking time in a yellow bean recombinant inbred inline population segregating for these traits.
Objective 4: Conduct In vivo feeding trial on the most diverse and best performing yellow bean genotypes from the Yellow Diversity Panel and the RIL (Recombinant Inbred Lines) population.
Approach:
1) A Yellow Bean Diversity Panel (YDP) will be established. The YDP will be comprised of 200 edible beige, yellow and brown dry bean genotypes originating from both the Mesoamerican and Andean genepools. Since yellow colored beans are present in both gene pools, we plan to characterize germplasm for both sources to capture the greatest diversity.
2) ARS Scientists will phenotype the Yellow Bean Diversity Panel for agronomic value, convenience, nutrition and taste. Seed of the 200 YDP lines plus 10 check lines of various market classes will be grown in replicated field trials in a randomized complete block design with two replications at the Michigan State University Montcalm Research Farm in Montcalm County, MI for two field seasons (2017, 2018) and in Isabela, Puerto Rico for one field season (2018). Nutritional studies of these harvests will be conducted at Ithaca, NY. Consumer acceptability studies will be conducted at Michigan State University.
3) ARS Scientists will phenotype and conduct QTL (Quantitative Trait Loci) mapping for iron bioavailability and cooking time in a yellow bean recombinant inbred inline population segregating for these traits. A recombinant inbred line population (RIL) F5:F7 of 240 lines has been developed to be used to identify QTL related to convenience, nutrition, and taste.
4) ARS Scientists in Ithaca, NY will conduct in vivo feeding trial on the most diverse and best performing yellow bean genotypes from the Yellow Diversity Panel and the RIL population. These studies will be designed to be the final confirmation of nutritional improvement and that lines are ready for release for farmers and consumers.
