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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Research Project #432603

Research Project: Inhibition of the Wheat Pathogen Fusarium graminearum by Endophytic Bacteria

Location: Mycotoxin Prevention and Applied Microbiology Research

Project Number: 5010-42000-048-01-N
Project Type: Non-Funded Cooperative Agreement

Start Date: Aug 1, 2017
End Date: Jul 31, 2018

Objective:
For bacteria associated with wheat heads, link in vitro assessment of functional traits to in situ ability to suppress Fusarium head blight development.

Approach:
Isolate bacteria from wheat tissue samples; aim for 35-50 unique strains (based on morphology and provisional taxonomic identification). Perform in vitro assessments of growth rate and interactions with Fusarium graminearum (antagonism, spore germination inhibition, competition for nutrients), under diverse conditions of temperature, osmotic potential, and CO2 enrichment. For the 15 bacterial strains encompassing the widest range of in vitro traits, perform in vivo tests of ability to inhibit Fusarium head blight (inoculations of wheat plants in growth chamber setting). Seek statistical linkages between in vitro trait assessment and disease inhibition parameters.