Project Number: 2040-22430-026-28-I
Project Type: Interagency Reimbursable Agreement
Start Date: Feb 15, 2017
End Date: Feb 14, 2019
1. Identify the genetic basis for black pupae in the SIT Mexfly Strain Tapachula-7 and Family-10. 2. Identify the genetic basis for differences in fecundity between Tapachula-7 and Family-10. 3. Characterize the translocation in Tapachula-7 and Family-10 that confers sex-linkage.
1. Develop mapping populations for A. ludens (Mexfly) and obtain genome-wide SNP fenotypes from a ddRAD-Seq library. 2. Generate a linkage map and perform a QTL analysis identifying the loci linked to black pupae and fecundity. 3. Use a sequence technique developed by 10x Genomics to identify structural variation between a wild type line and the two translocated SIT lines Tapachula-7 and Family-10.