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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Crop and Commodity Protection Research » Research » Research Project #432546

Research Project: Finding the Genetic Basis of Pupal Color and Fecundity in Anastrepha ludens Released for SIT Supporting Area Wide Suppression

Location: Tropical Crop and Commodity Protection Research

Project Number: 2040-22430-026-28-I
Project Type: Interagency Reimbursable Agreement

Start Date: Feb 15, 2017
End Date: Feb 14, 2019

Objective:
1. Identify the genetic basis for black pupae in the SIT Mexfly Strain Tapachula-7 and Family-10. 2. Identify the genetic basis for differences in fecundity between Tapachula-7 and Family-10. 3. Characterize the translocation in Tapachula-7 and Family-10 that confers sex-linkage.

Approach:
1. Develop mapping populations for A. ludens (Mexfly) and obtain genome-wide SNP fenotypes from a ddRAD-Seq library. 2. Generate a linkage map and perform a QTL analysis identifying the loci linked to black pupae and fecundity. 3. Use a sequence technique developed by 10x Genomics to identify structural variation between a wild type line and the two translocated SIT lines Tapachula-7 and Family-10.