Location: Crop Genetics Research
Project Number: 6066-22000-075-000-D
Project Type: In-House Appropriated
Start Date: Mar 4, 2017
End Date: Mar 3, 2022
Objective 1. Characterize new sources of reniform nematode resistance in Gossypium (G.) arboreum and G. herbaceum germplasm accessions and identify DNA markers associated with resistance. Objective 2. Introgress reniform nematode resistance from G. arboreum and G. herbaceum accessions into G. hirsutum and develop breeding lines with resistance. Objective 3. Determine effectiveness of unique sources of reniform resistance fromdiploid Gossypium germplasm accessions on nematode growth, reproduction, and infection. Objective 4. Characterize plant growth and development and yield responses to reniform nematode in susceptible and resistant cotton lines and define the relationships between soil fertility and reniform nematode severity with respect to plant damage and yield loss. Subobjective 4a. Characterize plant growth and development and yield responses to reniform nematode in susceptible and resistant cotton lines. Subobjective 4b. Define the relationships between soil fertility and reniform nematode severity with respect to plant damage and yield loss in susceptible and resistant cotton lines. Objective 5. Evaluate impacts of integrated reniform nematode management practices on cotton yield, quality and reniform nematode population densities. Subobjective 5a. Investigate the efficacy of new commercially-available nematicides on the management of the reniform nematode, cotton yield and cotton fiber quality. Subobjective 5b. Investigate the effect of rotation with non-host/poor-host crops on management of the reniform nematode and crop yield.
Develop populations by crossing resistant accessions with one or more Gossypium (G.) arboreum accessions classified as susceptible or highly susceptible. Ovule culture will be used for the introgression of resistance from G. arboreum and G. herbaceum accessions to G. hirsutum varieties. Gossypium accessions with high levels of resistance to reniform nematode will be evaluated in growth chamber experiments to measure the effects of the resistance on number of infections, rate of development of females after infection, and production of eggs. Classical growth and agronomic analysis will be conducted over two years under field conditions at Mississippi State University’s Delta Research and Extension Center in Stoneville, Mississippi. A nutrient response experiment will be conducted under controlled environmental conditions. The relative efficacy of new seed-applied and in-furrow nematicides against the reniform nematode will be evaluated on one susceptible and two resistant cotton lines in a field trial to be established in two naturally-infested sites in Stoneville, Mississippi. A field trial will be established in a reniform nematode infested site in Stoneville, Mississippi.