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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Research Project #432402

Research Project: Nanoparticle Microarray for High-throughput Chemical Phenotyping of Microbiomes

Location: Food Safety and Enteric Pathogens Research

Project Number: 5030-31320-004-18-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Nov 15, 2016
End Date: Nov 14, 2019

Objective:
The objectives of this research are to: 1) Screen a selected number of nanoparticles most effective for laser desorption ionization mass spectrometry (LDI-MS) analysis of the gut microbiome 2) Production of nanoparticle microarrays for high-throughput LDI-MS analysis 3) Application of the nanoparticle microarray LDI-MS for large scale metabolomics on gut microbiomes

Approach:
As a part of separate project in the ARS lab, 240 turkeys have been treated with and without antibiotics and microbiota membership and function are being characterized using 16S rRNA gene analysis and shotgun metagenomics, respectively. Metabolomic analysis will be performed on a subset of intestinal samples using a traditional LC-MS approach. These metabolomic datasets will be used to identify selected nano-particles (NP) to detect metabolites in the cooperator’s laboratory. After the metabolite NP microarray is developed by the cooperator, the ARS investigator will provide 150 turkey intestinal samples to evaluate the speed and sensitivity of the metabolite NP microarray for high-throughput analysis. The ARS investigator will apply computational methods to analyze microbial networks and co-occurrence of metabolites with antibiotic exposure.