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ARS Home » Midwest Area » Columbia, Missouri » Biological Control of Insects Research » Research » Research Project #432262

Research Project: Establishment of Honey Bee Cell Lines

Location: Biological Control of Insects Research

Project Number: 5070-22000-037-007-C
Project Type: Cooperative Research and Development Agreement

Start Date: Jan 2, 2017
End Date: Jan 1, 2019

Objective:
Develop cell lines from selected tissues and stages of the honey bee, Apis mellifera.

Approach:
Honey bee cell lines will be established using ARS’ standard protocols. Cell cultures will be initiated from whole animals and specific tissues, including ovaries, testes, fat bodies and nervous tissues. Cell culture media for initiating and maintaining honey bee cell lines will be optimized, including testing a variety of basal media, media cocktails and media supplements. A number of methods will be evaluated for tissue dissociation, including mincing, gently homogenizing and using enzymes, such as trypsin or collagenase. A variety of specialized cell culture flasks and plates (coated with different attachment factors) will be evaluated in order to determine which vessels produce optimal cell growth and attachment.