Location: Crop Bioprotection Research
Project Number: 5010-22410-019-04-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2018
End Date: Aug 31, 2019
The objective of this project is to evaluate the potential of beneficial bacteria to control plant diseases vectored by ambrosia beetles 1) in the laboratory and 2) in the field.
The approach to objective (1) will be to expose beetles to a simulated treatment of beneficial bacteria and determine if they can vector the bacteria into their galleries. A wood based artificial diet and growth media will serve as a simulated host plant to test the beetles. After treatment and returning the beetles to their simulated hosts, the galleries will be dissected, evaluated for fungal inhibition, the microbes isolated and identified, to determine if the beetles can vector enough beneficial bacteria to inhibit growth of the fungal symbiont. The approach to objective (2) will be to expose beetles to a simulated treatment of beneficial bacteria and determine how the bacterial antagonists impact the beetles. Infested trees will be monitored for disease symptoms and dissected after 30 days to determine the presence of pathogens and antagonists in the tree and inside the mycangia of any reproducing beetles.