Location: Endemic Poultry Viral Diseases Research
2018 Annual Report
Accomplishments
1. Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene therapy. A majority of these NDV vectors express only a single foreign gene through either an independent transcription unit (ITU) or an internal ribosomal entry site (IRES). In the present study, ARS researchers at Athens, Georgia, combined the ITU and IRES methods to generate a novel NDV LaSota strain-based recombinant virus vectoring the red fluorescence protein (RFP) and the green fluorescence protein (GFP) genes. Biological assessments of the recombinant virus, rLS/IRES-RFP/GFP, showed that it was slightly attenuated in vivo, yet maintained similar growth dynamics and viral yields in vitro when compared to the parental LaSota virus. Expression of both the RFP and GFP was detected from the virus-infected DF-1 cells by fluorescence microscopy. These data suggest that the rLS/IRES-RFP/GFP virus may be used as a multivalent vector for the development of vaccines and gene therapy agents.
Review Publications
Ji, Y., Liu, T., Du, Y., Cui, X., Yu, Q., Wang, Z., Li, Y., Zhu, Q. 2018. A novel genotype VII Newcastle disease virus vaccine candidate generated by mutation in the L and F genes confers improved protection in chickens. Veterinary Microbiology. 216:99-106. https://doi.org/10.1016/j.vetmic.2018.01.021.
Eldemery, F., Li, Y., Yu, Q., Van Santen, V., Toro, H. 2017. infectious bronchitis virus S2 of 4/91 expressed from recombinant virus does not protect against ark-type challenge. Acarology International Congress Proceedings. 61(3):397-401. https://doi.org/10.1637/11632-032017-ResNoteR.
Hu, H., Roth, J., Zsak, L., Yu, Q. 2017. Engineered newcastle disease virus expressing the F and G protein of AMPV-C confers protection against challenges in turkeys. Scientific Reports. 7:4025. http://doi:10.1038/s41598-017-04267-7.
Yu, Q. 2017. Metapneumovirus infections. In: Saif, Y.M., Toro H., editors. Diagnosis of Major Poultry Diseases. Zaragozo, Spain: Grupo Asis Biomedia, S.L. p.38-40.
Noh, J., Lee, D., Lim, T., Lee, J., Day, J.M., Song, C. 2018. Isolation and genomic characterization of a novel avian orthoreovirus in Korea, 2014. Archives of Virology. 163:1307-1316. https://doi.org/10.1007/s00705-017-3667-8.
Ji, Y., Liu, T., Liu, B., Yu, Q., Cui, X., Guo, F., Chang, H., Zhu, Q. 2017. Two single mutations in the fusion protein of Newcastle disease virus confer hemagglutinin-neuraminidase independent fusion promotion and attenuate the pathogenicity in chickens. Virology. 509:146-151. https://doi.org/10.1016/j.virol.2017.06.021.
Dimitrov, K.M., Afonso, C.L., Yu, Q., Miller, P.J. 2017. Newcastle disease vaccines- a solved problem or a continuous challenge? Veterinary Microbiology. 206:126-136. https://doi.org/10.1016/j.vetmic.2016.12.019.
