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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Research Project #431963

Research Project: Developing Soybean Cultivars and Germplasm with Increased Protein Quantity and Quality for the Midwest and South

Location: Soybean Genomics & Improvement Laboratory

Project Number: 8042-21000-275-44-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2016
End Date: Sep 30, 2018

Objective:
Produce high protein and high yielding soybean varieties and experimental lines from wild soybean germplasm, and develop technology to identify new genes from wild soybean for the increase of protein content in cultivated soybean and transfer to elite breeding lines.

Approach:
Based upon the analysis of the nearly 1,200 wild soybean accessions in the USDA Soybean Germplasm Collection that have been analyzed with more than 50,000 SNP DNA markers, a core collection of 81 wild soybeans was identified. F2 seeds were obtained by crossing the wild soybean accessions of the core subset with elite soybean cultivars by collaborators. The F7-derived recombinant inbred lines (RILs) from 18 of the cultivated x wild crosses will be generated and will be genotyped with BARCSoySNP5K/Soysnp50k BeadChips or sequencing. The RILs will be evaluated for protein content and other seed compositions in two years. The genotypic and phenotypic data will be used to identify loci that are associated with high protein content, high amino acid content and specific in wild accessions. The identified genes will be introduced to elite cultivated soybean by collaborators. The whole genome DNA sequence analysis of each of the wild soybean lines in the core subset as well as the random “control lines” from each of the 53 cultivated x wild soybean crosses were completed with the on-going funding support from USB. Highly selected lines will be tested by the collaborators to local and regional tests to identify high protein lines that are equivalent in yield the best cultivars available. Genetic genotyping data will be collected from these lines from each cultivated x wild soybean cross. The information will be used to identify regions of the genome that must be present in order to produce high protein and yielding lines. Subsequently, markers will be developed and be used to assist gene transfer in the breeding programs.