Project Number: 5020-21000-008-02-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Oct 1, 2016
End Date: Sep 30, 2018
The overall objectives of the multi-investigator proposal relate to the development of high sucrose, low-raffinose and low stachyose soybean lines. Research will define the value of this trait for feed soybeans, identify optimal germplasm for the trait through forward and reverse genetics, and characterize the physiology and environmental stability of the trait. A new goal for this funding cycle is to combine the low RFO trait with soybean oil traits and high protein levels in the seed, which will require the understanding of nutrient partitioning in the seed. This research addresses the identification of carbohydrate-modified germplasm through reverse genetics. From the soybean TILLInG population at West Lafayette we will identify mutation in up to 15 target genes that may be involved in the control of carbohydrate levels in soybean seeds.
From the TILLING population (NMU-induced single base changes in Williams-82) DNA pools, we will amplify up to 15 targeted genes that are likely to alter seed carbohydrate profile. Gene targets are selected in collaboration with the cooperators, and primers will be designed and tested for robust (and unique) amplification from pooled plant DNA. Amplification products will be sequenced from pooled DNA using high throughput sequencing. This approach should identify a large number (20 per gene) of single nucleotide polymorphisms (SNPs) in this mutant population, and we will select those that are most likely to affect gene function for isolation and characterization of carbohydrate levels and inheritance. “Perfect markers” will be designed, optimized, and used to isolate individual plants carrying the mutation of interest from pools.