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ARS Home » Research » Research Project #431683

Research Project: Investigating Sources of Fusarium Head Blight Resistance from Wheat and its Wild Relatives

Location: National Programs

Project Number: 0500-00053-004-27-G
Project Type: Grant

Start Date: Sep 6, 2016
End Date: Sep 5, 2021

Objective:
Project 1: The overall goal of the proposed research is to over-express the Fhb1 QTL from Sumai3 in wheat. The long term applications will be to deploy Fhb1 in popular wheat and barley cultivars to minimize losses due to FHB. Project 2: 1) To confirm if knock-down of Lpx3 in other wheat varieties can similarly enhance FHB resistance; and 2) To identify Lpx3 variants that confer resistance to FHB in wheat varieties that can be utilized by breeding programs.

Approach:
Project 1: Constructs for over-expressing Fhb1 gene will be developed in suitable vectors. Transformation of amenable wheat cultivars Bobwhite and Fielder will be done using particle bombardment method. The transformants will be analyzed for difference in resistance levels (percentage diseased spikelets and Fusarium damaged kernels) and DON content. Project 2: Several truncation mutants on chromosomes 4A, 4B and 4D have been identified in the hexaploid and tetraploid wheat varieties Cadenza and Kronos. After the isolation of homozygous mutants, crossing with other homeologous mutants will be done, and they will be tested for FHB phenotype in homozygous condition after background clearing.