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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Research Project #431637

Research Project: Metabolomic Analysis for Identification of Biological Functions Associated with Beet Necrotic Yellow Vein Virus in Sugarbeet

Location: Crop Improvement and Protection Research

Project Number: 2038-22000-018-04-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 20, 2016
End Date: Sep 30, 2019

Objective:
1. Compare the metabolome of a near isogenic lines of susceptible sugarbeet with that of sugarbeet with each of two resistance genes to Beet Necrotic Yellow Vein Virus (BNYVV) (Rz1 and Rz2) at specific time points in the infection process. 2. Through (a) examining differences in the resistant (Rz1Rz1 and Rz2Rz2) and susceptible (rz1rz1/rz2/rz2) sugarbeet metabolomes, and (b) comparison with existing knowledge of protein and gene expression changes associated with infection and resistance from previous studies, we will identify important pathways that may be critical to BNYVV infection of susceptible sugarbeet and for suppression of BNYVV in resistant sugarbeet.

Approach:
Plant growth and BNYVV treatments. Sugarbeet lines with closely related genetic backgrounds will be used; two which are resistant (Rz1Rz1 and Rz2Rz2 genes) and one susceptible (rz1rz1/rz2rz2) to BNYVV. Soil containing a well established isolate of BNYVV pathotype A established in a pure culture isolate of P. betae will be mixed with equal parts sterile builders sand and placed into new Styrofoam cups. Parallel studies will be performed with virus and vector-free (healthy) soil, as well as with soil containing only P. betae (no virus). For each sugar beet variety, 50 seeds will be planted per cup, with five cups per treatment, and grown in a growth chamber at 24oC with 16 hour days and approximately 220 uM m-1s-2 light until 3 and 6 weeks after sowing. At each time point, seedlings from all five cups for each treatment will be pooled, and foliar and root portions of the plant separated at the crown. Pooled root samples from each pot will be tested by ELISA to confirm BNYVV infection. Remaining roots from the same samples will be used for metabolite extractions. Metabolites will be extracted from each sample using standard methods. Metabolite extracts will be analyzed at Colorado State University, spectra mapped, and isomers identified. Results will elucidate compounds and hormones that are involved in rhizomania disease development.