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ARS Home » Plains Area » Fort Collins, Colorado » Center for Agricultural Resources Research » Agricultural Genetic Resources Preservation Research » Research » Research Project #431628

Research Project: Protection of the USDA/ARS Citrus Collection from Disease through Cryopreservation Technologies

Location: Agricultural Genetic Resources Preservation Research

Project Number: 3012-21000-015-003-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 15, 2016
End Date: Aug 31, 2022

The objective of this research is to protect the citrus genetic resource collection in Riverside, CA through security back-up of accessions in cryogenic facilities in Fort Collins, Colorado.

The citrus germplasm collections at Riverside, CA include about 1,500 accessions. Of these, we plan to process 350 of the 500 clonally propagated accessions that are pathogen-tested as ‘clean’ and are currently maintained in an NPGS insect-proof screenhouse in Riverside. A subset of recovered shoot tips will be tested for presence/absence of pathogens. Work will also involve investigation of the feasibility of cryopreserving seeds from non-indexed materials and the presence/absence of pathogens in embryo tissues.