Location: Plant Germplasm Preservation Research
Project Number: 3012-21000-016-01-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 15, 2016
End Date: Sep 14, 2021
The objective of this research is to protect the citrus genetic resource collection in Riverside, CA through security back-up of accessions in cryogenic facilities in Fort Collins, Colorado.
The citrus germplasm collections at Riverside, CA include about 1,500 accessions. Of these, we plan to process 350 of the 500 clonally propagated accessions that are pathogen-tested as ‘clean’ and are currently maintained in an NPGS insect-proof screenhouse in Riverside. A subset of recovered shoot tips will be tested for presence/absence of pathogens. Work will also involve investigation of the feasibility of cryopreserving seeds from non-indexed materials and the presence/absence of pathogens in embryo tissues.