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ARS Home » Plains Area » Fort Collins, Colorado » Center for Agricultural Resources Research » Plant Germplasm Preservation Research » Research » Research Project #431628

Research Project: Protection of the USDA/ARS Citrus Collection from Disease through Cryopreservation Technologies

Location: Plant Germplasm Preservation Research

Project Number: 3012-21000-016-01-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 15, 2016
End Date: Sep 14, 2021

Objective:
The objective of this research is to protect the citrus genetic resource collection in Riverside, CA through security back-up of accessions in cryogenic facilities in Fort Collins, Colorado.

Approach:
The citrus germplasm collections at Riverside, CA include about 1,500 accessions. Of these, we plan to process 350 of the 500 clonally propagated accessions that are pathogen-tested as ‘clean’ and are currently maintained in an NPGS insect-proof screenhouse in Riverside. A subset of recovered shoot tips will be tested for presence/absence of pathogens. Work will also involve investigation of the feasibility of cryopreserving seeds from non-indexed materials and the presence/absence of pathogens in embryo tissues.