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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Food Safety and Intervention Technologies Research » Research » Research Project #431610

Research Project: Effect of Non-Thermal Treatments on the Survival, Injury and Inactivation of S.enterica, E.coli 0157:H7 and L. monocytogenes on Produce

Location: Food Safety and Intervention Technologies Research

Project Number: 8072-41420-020-008-N
Project Type: Non-Funded Cooperative Agreement

Start Date: Oct 1, 2016
End Date: Sep 30, 2017

Objective:
1)Assess the effects of combinations of gamma irradiation and biocontrol treatment on the survival and inactivation of pathogenic bacteria on produce, 2) evaluate ATP (biolumesnescence) assay to quantify cell injury and death; and 3) determine the efficacy of antimicrobial biosurfactants /and GRAS compounds to inactivate foodborne pathogens on produce.

Approach:
Alternative intervention technologies for fresh or minimally processed foods will be improved using combinations of non-thermal treatments/hurdle approaches under investigation. Post-harvest microbiological studies will be conducted to determine survival, injury and inactivation of S. enterica, E. coli O157:H7, and L. monocytogenes on produce in replicated experiments. In-vitro and produce assays will be used to determine bacterial survival and inactivations relative to the untreated controls. The effects of inocula levels, storage temperatures, and time and combinations of treatments on antimicrobial efficacy will be used to improve alternative intervention measures on fresh produce.