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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Diet, Genomics and Immunology Laboratory » Research » Research Project #431475

Research Project: Studies on the Effects of I3C/DIM on AhR-mediated Pathway

Location: Diet, Genomics and Immunology Laboratory

Project Number: 8040-51530-057-009-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2016
End Date: Aug 12, 2020

To determine the role of Arylhydrocarbon receptor (AhR) in indole-3-carbinol (I3C) and 3’, 3’-Diindolylmethane (DIM) modulation of prostate cancer stromal cell such as macrophage.

A cell culture model will be used to examine whether I3C and DIM’s inhibitory effects on the cytokine IL-1 beta is mediated through the AhR-dependent pathway. The human monocytic cell will be exposed to the physiological concentration of I3C or DIM and then stimulated with LPS as inflammatory stimuli. The effects of I3C or DIM on the LPS-induced increase in IL-1 beta mRNA and the protein level will be determined. To determine the role of AhR a knockdown of the AhR expressed will be performed using siRNA and the effects of an AhR knockdown on I3C or DIM’s modulatory effects on IL-1 beta will be assessed at the mRNA and protein level.