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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » ABADRU » Research » Research Project #431429

Research Project: Development of Tools for the Pathological Characterization of Rift Valley Fever Virus Infection

Location: Arthropod-borne Animal Diseases Research

Project Number: 3020-32000-009-24-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2016
End Date: Aug 31, 2019

Objective:
Development and validation of novel in situ hybridization platforms for detection of Rift Valley fever (RVF) viral RNA in tissues. In the NP103 Animal Health Project related to bunyaviruses there is a goal to understand the mechanisms of viral pathogenesis, which will require the generation of necessary pathological tools.

Approach:
1. Develop high sensitivity in situ hybridization assays for detection of RVF viral RNA in tissues and compare them to real time RT-PCR and IHC. 2. Evaluate multiplex in situ hybridization’s potential to offer a DIVA compatible platform for RVFV\ viral RNA detection in tissues.