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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Research Project #431214

Research Project: Improved Sensitive Detection of the HLB Pathogen by New Gene Primers for Real Time and Droplet Digital PCR Analyses

Location: Crop Diseases, Pests and Genetics Research

Project Number: 2034-22000-013-06-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Jan 1, 2017
End Date: Sep 30, 2019

The research objective is to improve reliable diagnosis of "Candidatus Liberibacter asiaticus", presumptive causal agent of huanglongbing (HLB)in the U.S.

The approach includes deep sequencing using MiSeq Illumina to improve full length sequence data of 'Ca. Liberibacter asiaticus'; and bioinformatic analysis, employing primer design software, Taqman probes, real time quantitative PCR, droplet digital PCR, high throughput robotic DNA extraction and purification of nucleic acid.