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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Research Project #431214

Research Project: Improved Sensitive Detection of the HLB Pathogen by New Gene Primers for Real Time and Droplet Digital PCR Analyses

Location: Crop Diseases, Pests and Genetics Research

Project Number: 2034-22000-013-06-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Jan 1, 2017
End Date: Sep 30, 2019

Objective:
The research objective is to improve reliable diagnosis of "Candidatus Liberibacter asiaticus", presumptive causal agent of huanglongbing (HLB)in the U.S.

Approach:
The approach includes deep sequencing using MiSeq Illumina to improve full length sequence data of 'Ca. Liberibacter asiaticus'; and bioinformatic analysis, employing primer design software, Taqman probes, real time quantitative PCR, droplet digital PCR, high throughput robotic DNA extraction and purification of nucleic acid.