Project Number: 2034-22000-013-06-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Jan 1, 2017
End Date: Sep 30, 2019
The research objective is to improve reliable diagnosis of "Candidatus Liberibacter asiaticus", presumptive causal agent of huanglongbing (HLB)in the U.S.
The approach includes deep sequencing using MiSeq Illumina to improve full length sequence data of 'Ca. Liberibacter asiaticus'; and bioinformatic analysis, employing primer design software, Taqman probes, real time quantitative PCR, droplet digital PCR, high throughput robotic DNA extraction and purification of nucleic acid.