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ARS Home » Northeast Area » Kearneysville, West Virginia » Appalachian Fruit Research Laboratory » Innovative Fruit Production, Improvement, and Protection » Research » Research Project #431094

Research Project: Abiotic Stress and Adaptive Phenology in Fruit and Forest Trees

Location: Innovative Fruit Production, Improvement, and Protection

Project Number: 8080-21000-029-07-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Sep 1, 2016
End Date: Dec 14, 2018

Objective:
The objective for this project will be to understand gene function and mechanism in controlling dormancy and chilling requirement relevant to plant growth and development in fruit and forestry plants.

Approach:
Transgenic plum plants carrying either silenced or overexpressed genes that control the dormancy entry and release, as well as chilling requirement, will be evaluated for their dormancy behavior during the growth season, fall and winter in the greenhouse. The gene function in the chilling requirement will be evaluated by treating the vegetative buds at 40 degrees C for various times and then transferred to the greenhouse for examination of their chilling requirement. Those lines with abnormal dormancy behavior or altered chilling requirement will be further subjected to various molecular analyses (e.g. Q-RT-PCR, northern blot, small RNA production) to make sure that the changed phenotypes are brought about by the introduced transgenes.